Total Phenolic Content Values Research Articles

Phenolic Compounds Profile and Antioxidant Capacity of Plant-Based Protein Supplements

The study aimed to determine the phenolic content and antioxidant capacity of five protein supplements of plant origin. The content and profile of phenolics were determined using the UHPLC-DAD-MS method, while antioxidant capacity (ABTS and DPPH assays) and total phenolic content (TPC) were evaluated using spectrophotometric tests. In the analyzed proteins, twenty-five polyphenols were detected, including eleven phenolic acids, thirteen flavonoids, and one ellagitannin. Hemp protein revealed the highest individual phenolics content and TPC value (1620 μg/g and 1.79 mg GAE/g, respectively). Also, hemp protein showed the highest antioxidant activity determined via ABTS (9.37 μmol TE/g) and DPPH (9.01 μmol TE/g) assays. The contents of p-coumaric acid, m-coumaric acid, kaempferol, rutin, isorhamnetin-3-O-rutinoside, kaempferol-3-O-rutinoside, and TPC value were significantly correlated with antioxidant activity assays. Our findings indicate that plant-based protein supplements are a valuable source of phenols and can also be used in research related to precision medicine, nutrigenetics, and nutrigenomics. This will benefit future health promotion and personalized nutrition in the prevention of chronic diseases.

Characterization of Red Cabbage Extracts incorporated in Green Based Gelatin Active Films

This study aimed to assess red cabbage extracts obtained using solvents with different pH levels and alcohol concentrations, comparing their phytochemical quantities and functional characteristics. Solvents—purified water (PW, pH 7), 70% and 99% ethanol (70%E and 99%E, pH 6.5), and acidified ethanol (AE, pH 2)—were employed for extraction. When the different pH solutions were applied to the red cabbage extracts, they altered the extract's color, ranging from pink to yellow, influenced by various anthocyanin forms responsive to pH levels. The AE-extracted red cabbage displayed the highest UV and visible light barrier and total phenolic content (TPC). Then, the AE extract was used to formulate the green-based gelatin active films, alongside the 99%E extract, which had similar alcohol content and TPC values. The films averaged 20-21 μm in thickness. Moreover, incorporating both extracts into the gelatin films increased moisture content and water solubility, though the change in water vapor permeability was not significant compared to the neat gelatin film. The gelatin film incorporating AE-extracted red cabbage (GAE) showed higher water solubility than that incorporating the 99%E extract (GE). Both green-based gelatin active films showed differences in appearance color in the third week. The GAE film exhibited greater color stability, attributed to anthocyanin's distinctive chemical structures favoring acidic environments. This gelatin film incorporating red cabbage by AE-extracted holds promise as an eco-friendly active food packaging material.

Optimizing the drying conditions of date plum (Diospyros lotus L.) to conserve its phenolic content and antioxidants for preparing a highly bioaccessible polyphenol-rich tea

AbstractDate plum (Diospyros lotus L.) fruits are a good source of bioactive compounds and antioxidants. Drying can increase the shelf life of the fruit and its applications in the food development industry. Optimizing the drying conditions can help to produce prime-quality dried date plum fruits and conserve nutrients including phytochemicals. This study used a two-factor graphics-optimal design to optimize convective drying considering the air velocity and drying temperature of date plum fruits. The independent factors considered included drying temperature (43.78–86.21 °C) and air velocities (0.54–1.96 m/s), and the responses included total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging activity of date plum fruit. The optimized drying conditions (68 °C and 1.75 m/s) resulted in desirable TPC, TFC, FRAP, and DPPH values. The findings indicated that long drying time at low temperatures significantly decreased the phenolics and antioxidants. Date plum tea with different decoction times (5, 10, and 15 min) was prepared from fruits dried at optimum conditions. A decoction time of 5 min resulted in the highest catechin, vanillic, epicatechin, syringic acid, and quercetin-3-glucoside content, which were 2.45 ± 0.04, 11.06 ± 0.11, 22.03 ± 0.11, 12.95 ± 0.08, and 9.37 ± 0.10 mg/L; respectively. In vitro gastrointestinal digestion revealed that the tea product can be a source of highly bioaccessible (> 80%) gallic acid, catechin, vanillic acid, and quercetin-3-glucoside. Applying optimized drying conditions to dehydrate date plum fruit can be useful in preparing a highly bioaccessible polyphenol-rich tea.

Extraction and In Vitro Skincare Effect Assessment of Polysaccharides Extract from the Roots of Abelmoschus manihot (L.)

Obtaining high-added value compounds from agricultural waste receives increasing attention, as it can both improve resource utilization efficiency and reduce waste generation. In this study, polysaccharides are extracted from the discarded roots of Abelmoschus manihot (L.) by the high-efficiency ultrasound-assisted extraction (UAE). The optimized condition was determined as solid–liquid ratio SL ratio = 1:20, temperature T = 30 °C and time T = 40 min, achieving an extraction yield of 13.41%. Composition analysis revealed that glucose (Glc, 44.65%), rhamnose (Rha, 26.30%), galacturonic acid (GalA, 12.50%) and galactose (Gal, 9.86%) are the major monosaccharides of the extract. The extract showed a low degree of esterification (DE) value of 40.95%, and its Fourier-transform infrared (FT−IR) spectrum exhibited several characteristic peaks of polysaccharides. Inspired by the wide cosmetic applications of polysaccharides, the skincare effect of the extract was evaluated via the moisture retention, total phenolic content (TPC) quantification, 2,2-Diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging activity, anti-hyaluronidase and anti-elastase activity experiments. The extract solutions demonstrated a 48 h moisture retention rate of 10.75%, which is superior to that of commercially available moisturizer hyaluronic acid (HA). Moreover, both the TPC value of 16.16 mg GAE/g (dw) and DPPH-free radical scavenging activity of 89.20% at the concentration of 2 mg/mL indicated the strong anti-oxidant properties of the extract. Furthermore, the anti-hyaluronidase activity and moderate anti-elastase activity were determined as 72.16% and 42.02%, respectively. In general, in vitro skincare effect experiments suggest moisturizing, anti-oxidant, anti-radical and anti-aging activities of the A. manihot root extract, indicating its potential applications in the cosmetic industry.

Effect of different encapsulation techniques on the pH, thermal and storage stability of vitexin

In this study, vitexin was encapsulated in chitosan microparticles and phytosome nanoparticles, in order to enhance its stability and bioavailability. The effect of types of carrier agents on the pH stability, thermal stability and shelf-life stability of vitexin-loaded particles was investigated. The free vitexin (V) was degraded at pH 7 due to its lowest value of total phenolic content (TPC), whereas vitexin-loaded chitosan microparticles (V-CHM) and vitexin-loaded phytosome nanoparticles (V-PHN) could improve the stability of vitexin at pH 7. After heat treatment at 120 and 150 °C, the V-CHM and V-PHN gave higher release of TPC and antioxidant activity. V-CHM and V-PHN could prolong the release of TPC and antioxidant activity values of vitexin during storage time of 60 days. The results suggest that chitosan microparticles and phytosome nanoparticles has ability to overcome the limitation and enable the application of vitexin.

Lipid Quality Assessment of Virgin Coconut Oil Produced with Different Blanching Methods

Virgin Coconut Oil (VCO) is prone to oxidation due to the natural enzymes present in the coconut. Blanching has been studied to inactivate lipase and peroxidase enzymes that create deterioration in the product. Adding citric acid in hot water during blanching resulted in a higher quality product due to complexing agent properties to chelate metal substances and lowering the pH to reduce the enzymatic activities of the products. This research aims to analyze the effect of different blanching methods on VCO's lipid quality, antioxidant properties, and total phenolic content. Fresh coconut was subjected to water blanching at 95ºC for 5 minutes and 80ºC for 10 minutes with and without 0.05% citric acid addition. After that, VCO was produced using the chilling thawing method, which combines centrifugation and low-temperature incubation. VCO was analyzed in the free fatty acid (FFA), iodine value (IV), peroxide value (PV), TBARS value, the antioxidant activities by ABTS and DPPH, and total phenolic content (TPC). The results showed that water blanching with 0.05% citric acid at 80ºC for 10 minutes obtained better lipid quality compared to control with high IV (6.612±0.085 g iodine/g sample), low FFA (0.0241±0.0023 mg KOH/g sample), and low TBARS value (2.76±0.23 mg MDA/g sample). However, there is a contrary result between antioxidant activities and TPC that might reveal exciting findings. Despite a decrease in the antioxidant activities (DPPH: 39.93±3.11%RSA, ABTS: 9.80±0.91%RSA), the TPC value was increased (9.16±1.56 mg GAE/g sample). Therefore, hot water blanching with 0.05% citric acid addition can be conducted as a pre-treatment in the production of VCO to maintain the lipid quality, even though there is a contradiction in the TPC and antioxidant results.

Dehydration Impact on Antioxidant Potential and Phenolic content of Backhousia citrodora leaves

The most popular method for food preservation is dehydration. In order to enhance the overall quality and prolong the longevity of herbal products, it is imperative to carefully choose optimal dehydration conditions. The dehydration of Backhousia citriodora, also known as lemon myrtle leaves (LML), was conducted via three distinct techniques: conventional dehydration at temperatures of 40, 50, and 60°C (referred to as CD40, CD50, and CD60, respectively); vacuum dehydration at the same temperature as conventional dehydration with a pressure of 50 mbar; and heat pump dehydration at a constant temperature of 45°C. The antioxidant capacities, specifically the radical scavenging activity (DPPH) and ferric reducing antioxidant power (FRAP), along with the total phenolic content (TPC), were evaluated. HPD samples came up second to VD samples in terms of TPC retention, DPPH activity, and FRAP test, whereas CD samples had the lowest biochemical content across all dehydration conditions. The TPC and antioxidant activity in the CD sample exhibited a substantial reduction as the dehydration temperature increased. After dehydration, the CD60 sample had the largest reduction in TPC, DPPH, and FRAP values. Maximising the retention of biochemical content is of utmost importance in post-harvest processing as it serves as an indicator of greater retention. Therefore, the selection of appropriate dehydration techniques and conditions is critical in achieving this objective.

The Profile and Content of Polyphenolic Compounds and Antioxidant and Anti-Glycation Properties of Root Extracts of Selected Medicinal Herbs.

The objective of our study was to analyse the extracts from six medicinal herb roots (marshmallow, dandelion, liquorice, angelica, burdock, and comfrey) in terms of antioxidant capacity (ABTS, DPPH) and inhibition of advanced glycation end product (AGEs) formation. The quantification of phenolic acids and flavonoids was analysed using the UHPLC-DAD-MS method. Fifteen polyphenolic compounds were detected in the studied herbs. The higher number of polyphenols were found in marshmallows (ten polyphenols), while the lowest was in comfrey (five compounds). Liquorice root revealed the highest individual phenolic concentration (382µg/g dm) with the higher contribution of kaempferol-3-O-rutinoside. Comfrey root extract was characterised by the most abundant TPC (Total Phenolic Content) value (29.79mg GAE/ g dm). Burdock and comfrey showed the strongest anti-AGE activity studies with the BDA-GLU model. Burdock root was also characterised by the highest anti-AGE activity in the BSA-MGO model. The highest antioxidant capacity was determined by ABTS (72.12 µmol TE/g dw) and DPPH (143.01 µmol TE/g dw) assays for comfrey extract. The p-coumaric acid content was significantly correlated with anti-AGE activity determined by the BSA-MGO model. This research sheds new light on the bioactivity of root herbs, explaining the role of p-coumaric acid in preventing diabetes.

Characterization of multilayer bacterial cellulose-chitosan films loaded with grape bagasse antioxidant extract: Insights into spectral and water properties, microstructure, and antioxidant activity

This work explores the development and characterization of composite multilayer films comprising bacterial cellulose (BC) and chitosan enriched with antioxidant compounds from grape bagasse extract (GE) and glycerol. SEM images revealed a compact structure with successful interactions between BC and chitosan, confirmed by FT-IR analysis. Equilibrium moisture content, water vapor permeability (WVP), swollen capacity, and solubility were systematically investigated, unveiling the influence of glycerol and GE concentrations. Moisture content increased with elevated glycerol and GE levels, attributed to their hydrophilic nature. WVP rose with higher concentrations of hydrophilic compounds, affecting the films' permeability. Swollen capacity decreased, and solubility increased with the addition of GE and glycerol, indicating a more compact film structure. The incorporation of GE conferred antioxidant properties to the films, as evidenced by DPPH and ABTS+ assays, and Total Phenolic Content (TPC) determination. TPC values varied from 0 to 1.75 mg GAE/g dried film, depending on GE. Fourier Transform Infrared Spectroscopy (FT-IR) highlighted polymeric associations, and UV–Vis spectra demonstrated enhanced UV-blocking properties. Overall, these multilayer films offer promising applications in food packaging, leveraging natural antioxidant sources for an enhanced functionality.

Effect of drying methods on free and bound phenolic compounds, antioxidant capacities, and bioaccessibility of Cornelian cherry

AbstractCornus mas L. (Cornelian cherry, CM) fruits were dehydrated by solar-drying (SD) and freeze-drying (FD), and in addition to sugar and mineral contents, the free and insoluble-bound phenolics were determined in fresh and dried fruits. After subjecting the sample to simulated in vitro digestion, the change of free and bound phenolics at gastric and intestinal digestion steps was evaluated in fresh and dried CM fruits. In fresh CM fruits, the total phenolic content (TPC) was dominated by the bound fraction, whereas the contribution of free phenolics to the total content (free + bound) became more dominant (731–1439 mg GAE/100 g dw) in the dried fruits. The bioaccessibility (BI%) of TPC from fresh CM after digestion was 193%, whereas it was 18.60 and 48.02% for SD and FD fruits, respectively. The contribution free fraction to the total TPC value was around 28% in nondigested fresh samples and increased to 94% in digested samples; however, in dried samples, it was 64% prior to digestion and only increased to 70% in digested samples. A total of 17 phenolic compounds were identified in CM fruits: chlorogenic acid, caffeic acid, epicatechin, quercetin, cyanidin-3-O-glucoside, and pelargonidin 3-O-glucoside were only detected in the free fraction; gallic acid, vanillic acid, ferulic acid, and kaempferol were detected in higher amounts in the bound fraction. The quantity of detected phenolics in the nondigested sample generally decreased from the gastric to the intestinal stage of digestion. The release of phenolics from the fruit matrix and their degradation occurred simultaneously during digestion, and this could be affected by the state of the fruit, e.g., fresh or dried. Graphical abstract

Comparative study of different Malaysian Stingless bee propolis: Physicochemical characterization, Phytochemical contents and Antibacterial activity

Stingless bee propolis is known to contain a variety of bioactive compounds, including phenolic and flavonoids, which have been linked to its antibacterial properties. Nevertheless, the phytochemical compositions of stingless bee propolis are significantly influenced by a complex interplay of multiple factors such as geographical origin, floral source, and bee species. This study aimed to assess the physicochemical properties, phytochemical contents, and antibacterial activity of propolis from different stingless bee species found in the same environment and ecological system. The propolis samples obtained from Heterotrigona itama, Geniotrigona thoracica and Tetrigona apicalis were subjected to physicochemical analysis to determine the pH, moisture, lipid, resin, and wax contents. The total phenolic content (TPC) and total flavonoid content (TFC) were measured by Folin-Ciocalteu colorimetric and aluminium chloride methods, respectively. The antibacterial activity was determined using the agar well diffusion method against four Gram-positive bacteria, including Bacillus cereus, Micrococcus luteus, Streptococcus mutans, and Staphylococcus aureus. The physicochemical analysis of the propolis samples yielded the following results: moisture (12.17-16.45%), lipid (2.95-9.48%), resin (39.00-51.00%), wax (26.50-37.00%), and pH (5.07-5.61). Results revealed that propolis produced by G. thoracica displayed significantly higher moisture (16.45±0.38%), and resin contents (51.00±1.41%), as well as significantly lower percentage of lipid (2.95±0.12%), and wax content (26.50±0.71%). Similarly, G. thoracica propolis extract exhibited significantly higher TPC (200.70±0.06mg/mL GAE) and TFC (141.60±3.63mg/mL QE) values compared to the other propolis samples. Additionally, G. thoracica propolis extract was significantly active against B. cereus and M. luteus with inhibition zones of 15.00 and 16.00, respectively, and minimum inhibition concentration (MIC) of 390.63μg/mL. A strong correlation was found between resin content, TPC and antibacterial activity of propolis. This study indicated that the presence of high resin content in propolis resulted in a high concentration of phenolic compounds, which contributed significantly to its antibacterial activities. Furthermore, the research highlighted the species-dependent effect of propolis on its physicochemical characteristics, phytochemical composition, and antibacterial properties. The observed antibacterial efficacy of G. thoracica propolis suggested that the propolis extract held promise as an alternative treatment option against bacterial infections. Further research is warranted to fully elucidate the specific phenolic compounds that could contribute to its antibacterial properties.

The Effect of Drying Time on the Antioxidant Properties of Single Black Garlic Powder

Black garlic is a processed product derived from fresh garlic that generally has a taste and aroma that is less preferred. A drying process is employed to increase the attractiveness of consuming black garlic. This study aims to assess the impact of the drying time on the antioxidant activity and content of antioxidant compounds such as phenolics, flavonoids, and tannins in single black garlic. The drying time is expected to yield a single black garlic powder that is more convenient for consumption, whether in capsule form or as an addition to food products. With low moisture content, single black garlic powder has a longer shelf life and high antioxidant activity, potentially commanding a higher market value. In this research, the drying time was carried out on a single black garlic using an oven for 6, 12, 18, and 24 hours. The analysis of the total phenolic content (TPC), entire tannin content (TTC), and total flavonoid content (TFC) was determined using a Uv-Vis spectrophotometer, and each component was measured concerning gallic acid equivalent for TPC, quercetin for TFC, and tannic acid for TTC. Antioxidant activity was determined using the DPPH method, and the outcomes were expressed as IC50 values. The research shows that the extended drying time leads to an augmentation of the antioxidant compound in black garlic. Drying for 24 hours produces significant outcomes, showing potent antioxidant activity with an IC50 value of 37,019 ppm (powerful antioxidant category), total phenolic content value is 44,785 ± 0,033 mgGAE/g, total flavonoid content value is 32,033 ± 0,008 mgQE/g, and total tannins content value is 74,884 ± 0,057 mgTAE/g. Statistical analysis using a one-way ANOVA showed a significant influence of drying time on TPC, TFC, and TTC (p < 0,05). The longer the drying time, the higher the antioxidant activity and the content of compounds acting as antioxidants in black garlic powder.

Sustainable Valorization of Industrial Cherry Pomace: A Novel Cascade Approach Using Pulsed Electric Fields and Ultrasound Assisted-Extraction.

In this study, a two-stage cascade extraction process utilizing pulsed electric fields (PEF) (3 kV/cm, 10 kJ/kg) for initial extraction, followed by ultrasound (US) (200 W, 20 min)-assisted extraction (UAE) in a 50% (v/v) ethanol-water mixture (T = 50 °C, t = 60 min), was designed for the efficient release of valuable intracellular compounds from industrial cherry pomace. The extracted compounds were evaluated for total phenolic content (TPC), flavonoid content (FC), total anthocyanin content (TAC), and antioxidant activity (FRAP), and were compared with conventional solid-liquid extraction (SLE). Results showed that the highest release of bioactive compounds occurred in the first stage, which was attributed to the impact of PEF pre-treatment, resulting in significant increases in TPC (79%), FC (79%), TAC (83%), and FRAP values (80%) of the total content observed in the post-cascade PEF-UAE process. The integration of UAE into the cascade process further augmented the extraction efficiency, yielding 21%, 49%, 56%, and 26% increases for TPC, FC, TAC, and FRAP, respectively, as compared to extracts obtained through a second-stage conventional SLE. HPLC analysis identified neochlorogenic acid, 4-p-coumaroylquinic, and cyanidin-3-O-rutinoside as the predominant phenolic compounds in both untreated and cascade-treated cherry pomace extracts, and no degradation of the specific compounds occurred upon PEF and US application. SEM analysis revealed microstructural changes in cherry pomace induced by PEF and UAE treatments, enhancing the porosity and facilitating the extraction process. The study suggests the efficiency of the proposed cascade PEF-UAE extraction approach for phenolic compounds from industrial cherry pomace with potential applications to other plant-based biomasses.

Exploring the Chemical Profile, In Vitro Antioxidant and Anti-Inflammatory Activities of Santolina rosmarinifolia Extracts.

In this study, the phytochemical composition, in vitro antioxidant, and anti-inflammatory effects of the aqueous and 60% ethanolic (EtOH) extracts of Santolina rosmarinifolia leaf, flower, and root were examined. The antioxidant activity of S. rosmarinifolia extracts was determined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The total phenolic content (TPC) of the extracts was measured by the Folin-Ciocalteu assay. The anti-inflammatory effect of the extracts was monitored by the Griess assay. The chemical composition of S. rosmarinifolia extracts was analysed using the LC-MS technique. According to our findings, 60% EtOH leaf extracts showed the highest Trolox equivalent antioxidant capacity (TEAC) values in both ABTS (8.39 ± 0.43 µM) and DPPH (6.71 ± 0.03 µM) antioxidant activity assays. The TPC values of the samples were in good correspondence with the antioxidant activity measurements and showed the highest gallic acid equivalent value (130.17 ± 0.01 µg/mL) in 60% EtOH leaf extracts. In addition, the 60% EtOH extracts of the leaves were revealed to possess the highest anti-inflammatory effect. The LC-MS analysis of S. rosmarinifolia extracts proved the presence of ascorbic acid, catalpol, chrysin, epigallocatechin, geraniol, isoquercitrin, and theanine, among others, for the first time. However, additional studies are needed to investigate the direct relationship between the chemical composition and physiological effects of the herb. The 60% EtOH extracts of S. rosmarinifolia leaves are potential new sources of natural antioxidants and anti-inflammatory molecules in the production of novel nutraceutical products.

Total phenolic content and in vitro photoprotective activity of ethanolic extract of torch ginger (Etlingera elatior)

Background: Previous research highlighted the photoprotective potential of various extracts from torch ginger leaves, but the efficacy of ethanolic extracts, particularly from leaves and other plant parts, remained unexplored.
 Objective: This study aimed to assess the total phenolic content and SPF value of ethanolic extracts from torch ginger leaves, flowers, and stems, comparing these with a known photoprotective agent, oxybenzone.
 Method: Ethanolic extraction was performed on approximately 200 grams of each plant part. The total phenolic content was determined using the Folin-Ciocalteu method, while SPF values were measured spectrophotometrically between 290 and 320 nm. Statistical analysis involved Kruskal-Wallis tests followed by post-hoc Fisher's LSD.
 Results: The leaf extract exhibited the highest phenolic content (483.788 ± 2.57 mg GAE/g) and SPF value at 1000 ppm, surpassing that of flowers, stems, and the positive control, oxybenzone. The correlation between phenolic content and SPF value across plant parts was statistically significant.
 Conclusion: The ethanolic extract of torch ginger leaves shows superior photoprotective potential, indicated by its high phenolic content and SPF value, suggesting its promising application in natural sunscreen formulations up to the permissible active substance limit of 6% set by BPOM.

In Vitro Determination of Nitric Oxide Synthase Inhibition, Antioxidant Capacity and Phenolic Content of Various Natural Products (Bee and Herbal Products).

It is obvious that the oxidation process is an undeniable fact and when it comes to aging, one of the first solutions that come to mind is natural products. When it comes to natural products, both plants and bee products play an important, almost combative role against oxidation. For this purpose, natural products of both plant and animal origin were considered together in our study: Linden, green tea, aronia, wild grapes, myrtle, blueberries and basil, honey, pollen and propolis. Total phenolic content values of the extracts ranged between 49.28 and 3859.06 mg gallic acid equivalent/100 g, and propolis, green tea, chestnut flower and aronia samples were found to have the highest values. When looking at the NOS inhibition potential, it was determined that propolis, pollen and aronia samples had the highest percentage inhibition values of 98.11, 92.29, 83.44, respectively. Antioxidant activities of methanolic extracts were investigated using iron(III) reducing/antioxidant capacity (FRAP), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity test and NOS inhibition tests. The phenolic composition of methanolic extracts was tested using the RP-HPLC-UV (high-performance liquid chromatographic method with ultraviolet) method with 19 phenolic standards.

Effect of industrial steam distillation conditions on volatiles and antioxidant capacity of Cananga odorata essential oils

ABSTRACT The ylang-ylang (Cananga odorata) essential oil is valued for its medicinal and cosmetic benefits. This study compared the volatile compounds and antioxidant capacity of six essential oils from C. odorata flowers obtained industrially by steam distillation. The volatiles were identified by gas chromatography-mass spectrometry (GC/MS). The ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and total phenolic content (TPC) were measured by ultraviolet-visible (UV-Vis) spectrophotometry. The main components included benzyl acetate (21.78–27.82%), benzyl benzoate (12.30–18.07%), linalool (9.45–14.39%), and germacrene D (9.17–14.46%). The highest antioxidant values were shown by the sample S6 (206.50 ± 7.45 mmol TE/L, 52.03 ± 0.88 mg GAE/g), followed by S5. The multivariate analysis separated S6 and S5 from the remaining essential oils, suggesting that extraction was more influenced by the steam flow rate. Notably, the antioxidant capacity of S6 revealed a positive correlation between volatiles and TPC values. These findings denote the importance of improving empirical industrial procedures.

Physicochemical and microbiological characteristics of kefir ice cream with the addition of bengkuang (Pachyrhizus erosus (L.)) starch

The technology for fermenting milk kefir and the development in processing kefir ice cream is crucial to produce superior food products that support health. The addition of prebiotics from bengkuang (Pachyrhizus erosus (L.)) starch in making ice cream is the main purpose of improving the quality of kefir ice cream. This research purposed to determine the physicochemical (moisture, ash, protein, fat, phenolics, and DPPH for free radical scavenging activity) and microbiological (lactic acid bacteria, yeast, Lactobacillus, Lactococcus, Leuconostoc) characteristics of kefir ice cream after the addition of 3, 5, 7 and 9% (w/w) bengkuang starch. This ice cream used fresh cow’s milk, skim milk, kefir, granulated sugar, whipped cream, CMC (Carboxy Methyl Cellulose), egg yolks, and varying amounts of bengkuang starch. The research showed that the best formulation for all physicochemical and microbiological characteristics was the kefir ice cream added with 9% (w/w) bengkuang starch. This product had stable viability of probiotic microbes (both lactic acid bacteria and yeast), the same protein value as the control, low fat, pH suitable for probiotics, high total phenolic content and DPPH value, and low melting rate even though the overrun value was lower compared to controls. The synergistic combination of bengkuang starch and probiotics from kefir in making ice cream has the potential to produce nutritious functional foods that are beneficial for human health.

Effects of pretreatment and drying temperatures on physicochemical and antioxidant properties of dried duku (Lansium domesticum)

Duku (Lansium domesticum) is a native of Southeast Asia. Duku is a seasonal fruit and highly deteriorated due to high moisture content of more than 80 %, thus difficult to commercialise due to the short shelf life. The transformation of fresh into dried fruit will increase the commercial values as well as be a solution to reduce fruit loss. This study was conducted to evaluate the effects of pretreatments and drying temperatures on physicochemical and antioxidant properties in dried duku fruit. The duku fruits (whole, flesh, peels and seeds) were subjected to pretreatment process with ascorbic acid solution, sodium metabisulfite solution, sodium erythorbate solution and blanching. Then, the samples were dried at 50 °C, 60 °C, 70 °C and 80 °C, respectively. Determination of total phenolic content (TPC) was carried out based on the Folin-Ciocalteau method. The antioxidant activities were evaluated using ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The pH values of all parts of duku were decreased as the drying temperature increases. Seed (pretreated with sodium metabisulfite, dried at 80 °C) showed the highest total phenolic content value with 116.84 ± 0.31 mg gallic acid equivalents per g fruit extract (GAE/g), while lowest in flesh (pretreated with ascorbic acid, dried at 50 °C). Seed (pretreated with sodium metabisulfite, dried at 50 °C) has the highest antioxidant capacity with IC50 value of 41.15 µg/ml (ABTS free radical scavenging) and 121.35 µg/ml (DPPH free radical scavenging) compared to other fruit parts, pretreatments and drying temperatures. The findings of this study indicate the potential of all parts of duku fruit as a valuable source of nutrients. Information on the nutritional and antioxidant properties of this Malaysia's seasonal fruit reveals its potential to be commercialized into different food and beverage products.

Phytochemical Profile and Evaluation of the Antioxidant, Cyto-Genotoxic, and Antigenotoxic Potential of Salvia verticillata Hydromethanolic Extract.

This study comprises the phytochemical characterization, the evaluation of the total phenolic content (TPC) and antioxidant activity (AA), and the investigation of the cyto-genotoxic and antigenotoxic potential of hydromethanolic extract derived from Salvia verticillata L. leaves. HPLC-DAD-ESI-MS and HPLC-DAD were used for the characterization of the extract and determination of the major ingredients. Afterwards, the TPC and AA were determined. The cytotoxic and genotoxic effect of the extract on cultured human lymphocytes at concentrations of 10, 25, and 50 μg mL-1 was investigated via the Cytokinesis Block MicroNucleus (CBMN) assay. Moreover, its antigenotoxic potential against the mutagenic agent mitomycin C (MMC) was assessed using the same assay. The hydromethanolic extract comprises numerous metabolites, with rosmarinic acid being the major compound. It had a high value of TPC and exerted significant AA as shown by the results of the Ferric Reducing Antioxidant Power (FRAP) and Radical Scavenging Activity by DPPH• assays. A dose-dependent cytotoxic potential was recorded, with the highest dose (50 μg mL-1) exhibiting statistically significant cytotoxicity. None of the tested concentrations induced significant micronuclei (MN) frequencies, indicating a lack of genotoxicity. All tested concentrations reduced the MMC-mediated genotoxic effects, with the two lowest showing statistically significant antigenotoxic potential.